X-GAL CAS: 7240-90-6 Theko ea Moetsi

Phetoho ea 'Mala: X-Gal hangata ha e na 'mala empa, ha hydrolysis e etsoa ke β-galactosidase, e fetoha boputsoa.Phetoho ena ea 'mala e lumella ho lemoha le ho lekanya tšebetso ea β-galactosidase.

LacZ Gene Detection: X-Gal e sebelisetsoa ho khetholla lisele kapa liphatsa tsa lefutso tse hlahisang lefutso la lacZ.LacZ e sebelisoa hangata joalo ka moqolotsi oa liphatsa tsa lefutso ho baeloji ea limolek'hule ho lekola liphatsa tsa lefutso kapa ts'ebetso ea motšehetsi oa thuto.

Tlhahlobo ea Colony: X-Gal e sebelisoa hangata litekong tsa tlhahlobo ea kolone ea baktheria.Likokoana-hloko tsa baktheria tse bontšang LacZ li bonahala li le putsoa ha li lengoa ho agar e nang le X-Gal, e leng se nolofalletsang ho tsebahatsa le ho khetha likolone tse nang le lacZ-positive.

Tlhahlobo ea Gene Fusion: X-Gal e boetse e sebelisoa litekong tsa fusion fusion.Ha jini e shebiloeng e hokahane le lefutso la lacZ, litšila tsa X-Gal li ka senola mokhoa oa polelo oa protheine ea fusion ka har'a sele kapa lisele.

Protein Localization: X-Gal staining e ka sebelisoa ho etsa lipatlisiso tsa sebaka sa protheine ea subcellular.Ka ho kopanya protheine e khahlisang mofuta oa lacZ, ts'ebetso ea β-galactosidase e ka bonts'a moo protheine e leng teng ka har'a sele.

X-Gal Analogues: Mefuta e fetotsoeng ea X-Gal, joalo ka Bluo-Gal kapa Red-Gal, e entsoe ho lumella merero e meng ea ntlafatso ea mebala.Li-analogue tsena li etsa hore ho be le phapang lipakeng tsa lisele tsa lacZ-positive le lacZ-negative kapa lisele tse sebelisang mebala e fapaneng.