Tris(hydroxymethyl)nitromethane CAS:126-11-4

Phello:

Bokgoni ba ho Buffer: Tris e sebetsa e le moemeli ya sebetsang hantle wa ho buffer ka lebaka la bokgoni ba yona ba ho amohela kapa ho fana ka diprothone, e boloka mefuta e tsitsitseng ya pH ditharollong. E sebediswa hangata e le karolo ya mantlha ditsamaisong tsa buffer ho tsitsisa pH ya disampole tsa baeloji le dikarabelo.

Likopo:

Biology ea Limolek'hule: Tris e sebelisoa haholo e le sesebelisoa sa ho thibela likokoana-hloko mekhoeng e fapaneng ea baeloji ea limolek'hule, ho kenyeletsoa ho aroloa ha DNA le RNA, PCR, gel electrophoresis, le tlhoekiso ea protheine. E thusa ho boloka tikoloho e tsitsitseng ea pH, e lumellang maemo a matle bakeng sa likarabelo tsa enzymatic le likamano tsa limolek'hule.

Tloaelo ea Sele: Tris e atisa ho sebelisoa mecheng ea litaba tsa setso sa sele ho boloka pH e tsitsitseng le ho leka-lekana ha osmotic, ho tšehetsa kholo ea lisele tse phetseng hantle le ho phela hantle.

Khemistri ea Protheine: Tris e sebelisoa litekong tsa k'hemistri ea protheine, tse kang ho qhibiliha ha protheine, liteko tsa botsitso ba protheine, le lithuto tsa ho tlama protheine le ligand. E thusa ho boloka mefuta ea pH e lakatsehang, ho netefatsa hore protheine e mena le ho sebetsa hantle.

Enzymology: Tris e sebelisoa litekong tse fapaneng tsa enzymatic ho ntlafatsa maemo a pH a hlokahalang bakeng sa ts'ebetso ea enzymatic. E netefatsa liphetho tse tšepahalang le tse ka ikatisang, e nolofalletsang tekanyo e nepahetseng ea kinetics ea enzyme le lithuto tsa thibelo.

Liteko tsa Biochemical: Tris e sebelisoa e le karolo litekong tse ngata tsa biochemical ka lebaka la thepa ea eona ea ho thibela. E boloka pH e sa fetoheng nakong ea liteko tsa colorimetric, spectrophotometric, le enzymatic, e ntlafatsang ho nepahala le ho tšepahala ha liphetho.