Tris(hydroxymethyl)aminoethane CAS:77-86-1

Tris (hydroxymethyl)aminoethane e sebelisoa haholo lits'ebetsong tse fapaneng tsa indasteri le tsa meriana ka lebaka la thepa ea eona e feto-fetohang. Indastering ea meriana, e sebetsa e le moemeli oa buffering le mohlophisi oa pH ho thehoeng ha meriana e fapaneng le mekhoa ea meriana. Bokhoni ba eona ba ho boloka botsitso ba pH bo kenya letsoho katlehong le botsitsong ba lihlahisoa tsa meriana. Ho feta moo, motsoako ona o sebelisoa e le reagent lits'ebetsong tsa biochemistry le baeloji ea limolek'hule. E sebetsa e le amine ea mantlha, e etsa metsoako e nang le li-ion tsa tšepe 'me e sebetsa e le karolo ea bohlokoa litekong tse fapaneng tsa biochemical le liprothokholing tsa lipatlisiso. Sebakeng sa tlhoekiso ea protheine le chromatography, tris (hydroxymethyl)aminoethane e sebelisoa e le karolo ea buffer. Bokhoni ba eona ba buffering le ho lumellana ha lik'hemik'hale ho e etsa hore e lokele ho boloka maemo a tsitsitseng a pH nakong ea lits'ebetso tsa ho itšehla thajana le ho hloekisa protheine, ho netefatsa botšepehi le ts'ebetso ea limolek'hule. Ho feta moo, e sebetsa e le karolo mecheng ea temo ea sele, e fana ka tikoloho e tsitsitseng bakeng sa kholo le phatlalatso ea lisele libakeng tsa laboratori. Karolo ea eona ho bolokeng maemo a pH a matle e kenya letsoho bophelong bo botle le ho phela hantle ha lisele tse lengoang tse sebelisoang lipatlisisong tsa baeloji le tsa bongaka. Ha ho etsoa lihlahisoa tsa tlhokomelo ea botho le tsa litlolo, tris (hydroxymethyl)aminoethane e sebetsa e le motsoako mefuteng e kang lihlahisoa tsa tlhokomelo ea moriri, litlolo tsa letlalo le litlolo. Thepa ea eona e mengata e thusa ho tsitsisa sehlahisoa, ho fetola pH, le ts'ebetso e akaretsang lits'ebetsong tse fapaneng tsa litlolo. Ho feta moo, e sebelisoa tlhahisong ea lik'hemik'hale tse ikhethang le lintho tse ling tse sebelisoang lits'ebetsong tse fapaneng tsa indasteri. Ho sebetsa ha eona ka lik'hemik'hale le ho lumellana ha eona le metsoako e meng ho e etsa hore e be molemo bakeng sa ho theha mekhoa e ikhethileng e etselitsoeng litlhoko tse itseng tsa indasteri. Ho feta moo, tris (hydroxymethyl)aminoethane e sebelisoa ho kopanyeng metsoako ea khokahano le li-catalyst tse sebelisoang tlhahisong ea lik'hemik'hale le meriana. K'hemistri ea eona ea khokahano le thepa ea ligand li kenya letsoho nts'etsopele ea lik'hemik'hale tse ikhethileng le litsamaiso tsa catalyst bakeng sa lits'ebetso tsa indasteri. Ka kakaretso, lits'ebetso tse fapaneng tsa tris (hydroxymethyl)aminoethane li kenyelletsa meriana, biochemistry, setso sa sele, litlolo, lik'hemik'hale tse ikhethang le lits'ebetso tsa indasteri. Thepa ea eona e feto-fetohang e thusa ho boloka botsitso ba pH, ho nolofatsa liketso tsa biochemical, ho ntlafatsa ts'ebetso ea sehlahisoa, le ho nolofalletsa tlhahiso ea lisebelisoa tse tsoetseng pele liindastering tse ngata.