Streptavidin ho tloha ho Streptomyces avidinii CAS:9013-20-1

Kamano e tlamang: Streptavidin e na le kamano e phahameng haholo bakeng sa biotin, e leng se etsang hore e be sesebelisoa se setle sa ho hapa le ho lemoha limolek'hule tse nang le biotinyle.

Ho hlwekisa protheine: Streptavidin hangata e sebediswa e le sesebediswa sa ho hlwekisa theknolojing ya baeloji. Di-tag kapa dimolek'hule tse nang le biotinyle di ka hokelwa ho di-streptavidin beads, e leng se dumellang ho arolwa le ho hlwekiswa ha diprotheine tse itseng ho tswa metswakong e rarahaneng.

Liteko tsa ho lemoha: Streptavidin e sebelisoa hangata litekong tsa ho itšireletsa mafung, joalo ka liteko tsa immunosorbent tse amanang le enzyme (ELISA), moo e ka tlamang li-antibodies kapa li-antigen tse lemohang tsa biotinylated. Sena se lumella ho lemoha ka hloko le ka ho khetheha liprotheine kapa li-biomarker tse fapaneng.

Phano ea meriana e reretsoeng: Streptavidin e ka sebelisoa lits'ebetsong tsa phano ea meriana ka ho e kopanya le metsoako ea phekolo kapa li-nanoparticles. Limolek'hule tse reretsoeng biotinylated li ka hokelloa ho streptavidin, e leng se nolofalletsang phano e reretsoeng ho ea liseleng kapa liseleng tse itseng.

Mekhoa ea baeloji ea limolek'hule: Streptavidin e sebelisoa mekhoeng e fapaneng ea baeloji ea limolek'hule, joalo ka liteko tsa nucleic acid hybridization le polymerase chain reaction (PCR). Li-probe kapa li-primer tse entsoeng ka biotinyle li ka sebelisoa ho lemoha kapa ho matlafatsa li-nucleic acid tse itseng, 'me streptavidin e sebelisoa ho bona kapa ho hapa lihlahisoa tse entsoeng ka biotinyle.

Ho nka ditshwantsho: Streptavidin hangata e sebediswa e le sesebediswa mekgweng ya microscopy ya fluorescence le ya ho nka ditshwantsho. Ka ho kopanya didae tse fluorescent le streptavidin, dipheo tse itseng di ka ngolwa le ho bonwa ka mahlo a kelello le qeto e phahameng.

Tlhahlobo: Liteko tse thehiloeng ho Streptavidin li sebelisoa haholo tlhahlobong ea bongaka bakeng sa ho lemoha mafu kapa likokoana-hloko. Li-probe tse nang le biotinylated li ka lemoha li-nucleic acid kapa li-antigen tse itseng, tse nolofalletsang ho hlahloba le ho beha leihlo maemo a fapaneng.