Proteinase K bakeng sa albamo ea tritirachium CAS:39450-01-6

Proteinase K ke enzyme e ntšitsoeng ho fungus album ea Tritirachium. Ke protease ea serine e ikhethang ka bophara, ho bolelang hore e ka arola litlamo tsa peptide liprotheineng tse ngata tse fapaneng. Proteinase K e tsebahala ka bokhoni ba eona ba ho senya liprotheine ka katleho, e leng se etsang hore e be sesebelisoa sa bohlokoa lits'ebetsong tse fapaneng.

E 'ngoe ea lits'ebetso tse ka sehloohong tsa Proteinase K ke lipatlisisong tsa baeloji ea limolek'hule. E sebelisoa hangata bakeng sa liprotheine tsa ho ntša DNA le RNA ho tlosa liprotheine tse ka sitisang tlhahlobo e latelang. Proteinase K e thusa ho qhibilihisa lisele le ho senya liprotheine tsa sele, e leng se lumellang ho aroloa ha li-nucleic acid tsa boleng bo holimo.

Proteinase K e boetse e sebelisoa haholo lithutong tsa tlhahlobo ea protheine le tsa tlhaloso. E ka sebelisoa bakeng sa tšilo ea protheine, e lumellang tlhahiso ea likaroloana tsa peptide tse ka hlahlojoang ka mekhoa e kang mass spectrometry. Ho feta moo, Proteinase K e thusa bakeng sa ho latellana ha protheine le ho khetholla boemo ba li-amino acid ka har'a ketane ea polypeptide.

Tšebeliso e 'ngoe ea bohlokoa ea Proteinase K ke ho hloekisa libaka tse ka holim'a tsona. E sebelisoa hangata lilaboratoring tsa baeloji ea limolek'hule ho tlosa li-nucleic acid, liprotheine le litšila tse ling tse tsoang lisebelisoa tsa laboratori le libakeng tse ka holimo. Proteinase K e tsitsitse haholo ebile e sebetsa tlas'a maemo a fapaneng, ho kenyeletsoa le mocheso o phahameng le maemo a fapaneng a pH.