5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ke motsoako o sebelisoang lithutong tse fapaneng tsa biochemical, haholo-holo bakeng sa ho lemoha le ho bona tšebetso ea enzyme.Ke substrate e ka kenngoa ka hydrolyzed ka li-enzyme tse itseng, e leng se bakang ho lokolloa ha sehlahisoa se mebala kapa sa fluorescent.
Motsoako ona o sebelisoa hangata litekong tsa ho bona boteng le tšebetso ea li-enzyme tse kang beta-galactosidase le beta-glucuronidase.Li-enzyme tsena li qhekella lihlopha tsa acetyl le glucosaminide ho tloha substrate, e leng se lebisang ho thehoeng ha chromophore e putsoa kapa e tala.
Sebopeho se ikhethileng sa 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide se lumella ho lemoha habonolo le ho lekanya mosebetsi oa enzyme.Tšebeliso ea eona mekhoeng e fapaneng ea liteko, ho kenyelletsa histochemistry, immunohistochemistry, le litlhahlobo tse thehiloeng liseleng, e tlatselitse kutloisisong e ntle ea mesebetsi ea enzyme.