PMSF CAS:329-98-6 Theko ea moetsi

Phello:

PMSF ke sethibela-protease sa serine se tlamang sebakeng se sebetsang sa serine proteases, ka hona se thibela ts'ebetso ea tsona.

E fetola masalla a serine ka dibopeho tsa covalent, e leng se etsang hore enzyme e se sebetse.

PMSF e ka sireletsa liprotheine hore li se ke tsa senyeha ka ho thibela ts'ebetso ea protease, haholo-holo serine proteases.

Likopo:

Patlisiso ea lik'hemik'hale tsa 'mele: PMSF e sebelisoa hangata nakong ea mekhoa ea ho hloekisa protheine le ho e arola ho thibela ho senyeha ha protheine. E thusa ho boloka botšepehi le botsitso ba protheine e reriloeng.

Thibelo ea Enzyme: PMSF ke sesebelisoa sa bohlokoa sa ho thibela liprotheine tsa serine nakong ea liteko tsa lienzyme kapa lithuto tsa biochemical. Ka ho thibela tšebetso ea liprotheine tsena, bafuputsi ba ka batlisisa karolo ea liprotheine tsa serine lits'ebetsong tse fapaneng tsa baeloji.

Lithuto tsa proteolysis: PMSF e ka sebelisoa ho laola kapa ho fetola ts'ebetso ea protease ho lysates ea sele kapa li-extracts. Sena se molemo bakeng sa ho ithuta liketsahalo tsa proteolytic tse amehang lits'ebetsong tsa sele kapa ha proteolysis e hloka ho laoloa ho setup ea liteko.

Thibelo e akaretsang ea protease: Le hoja PMSF e shebane ka ho khetheha le li-protease tsa serine, e ka boela ea ba le phello e itseng ea thibelo ho li-protease tse ling tsa cysteine. E sebelisoa e le thibelo e akaretsang ea protease ha ho sebelisoa lisampole tsa protheine ho fokotsa ho senyeha ha proteolytic.