P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Ho sibolloa ha tšebetso ea beta-galactosidase: PNPG e sebelisoa hangata litekong ho lekanya tšebetso ea beta-galactosidase, enzyme e susumetsang hydrolysis ea lactose hore e be tsoekere le galactose.Hydrolysis ea PNPG ka beta-galactosidase e hlahisa molek'hule ea p-nitrophenol (pNP), e ka bonoang spectrophotometrically ka lebaka la 'mala oa eona o mosehla.

Tlhahlobo bakeng sa li-enzyme inhibitors le activators: PNPG e ka sebelisoa tlhahlobong e phahameng ea ho tseba metsoako e fetolang ts'ebetso ea beta-galactosidase.Ka ho lekanya sekhahla sa PNPG hydrolysis boteng ba metsoako e fapaneng ea liteko, bafuputsi ba ka tseba li-inhibitors tse fokotsang ts'ebetso ea enzyme kapa li-activator tse ntlafatsang ts'ebetso ea enzyme.

Boithuto ba enzyme kinetics: Hydrolysis ea PNPG ka beta-galactosidase e latela Michaelis-Menten kinetics, e lumellang bafuputsi ho fumana lintlha tsa bohlokoa tsa enzyme joalo ka lebelo le phahameng la karabelo (Vmax) le Michaelis constant (Km).Tlhahisoleseding ena e thusa ho utloisisa kamano ea enzyme ea substrate le ts'ebetso e sebetsang hantle.

Ts'ebeliso ea baeloji ea limolek'hule: Beta-galactosidase, e arolang PNPG, e sebelisoa hangata joalo ka mofuta oa motlalehi ho baeloji ea limolek'hule.Karolo e nyenyane ea PNPG e atisa ho sebelisoa ho lemoha le ho bona ka mahlo polelo ea moqolotsi oa litaba, e fana ka mokhoa o bonolo le o hlokolosi oa ho hlahloba liphatsa tsa lefutso tsamaisong e fapaneng ea liteko.