NSP-SA-NHS CAS:199293-83-9 Theko ea moetsi

NSP-SA-NHS, e tsejoang hape e le N-succinimidyl S-acetylthioacetate N-hydroxysuccinimide ester, ke motsoako o sebelisoang hangata e le reagent e kopanyang e ikhethileng ea thiol liketsong tsa bioconjugation. Phello ea eona e kholo ke ho thehoa ha litlamo tse tsitsitseng tsa thioester pakeng tsa lihlopha tsa thiol tse teng holim'a limolek'hule, tse kang liprotheine kapa li-peptide.

Tšebeliso ea NSP-SA-NHS haholo-holo e sebetsa lefapheng la phetoho ea protheine le ho e thibela ho sisinyeha. Tse ling tsa lits'ebetso tsa eona tsa bohlokoa li kenyelletsa:

Ho ngolwa ha protheine: NSP-SA-NHS e sebediswa ho hokela dileibole ka kopanelo, jwalo ka didae tse fluorescent kapa biotin, ho diprotheine kapa dipeptide. Sena se dumella ho lemoha, ho hlwaya le ho latela di-biomolecule tse ngotsweng ditekong tse fapaneng tsa baeloji.

Tšebelisano-'moho ea protheine le protheine: NSP-SA-NHS e ka sebelisoa ho hokahanya liprotheine tse sebelisanang ho ithuta likamano-'moho ea protheine le protheine. Sena se atisa ho sebelisoa mekhoeng e kang co-immunoprecipitation kapa liteko tsa ho hula ho khetholla balekane ba tlamang kapa li-complexes tsa liprotheine tse ithutoang.

Ho thibela protheine ho tsamaya: NSP-SA-NHS e dumella ho hokela diprotheine kapa dipeptide ka covalent hodima dibaka tse tiileng, ho kenyeletswa le dibead tsa agarose, dibead tsa makenete, kapa di-microplate. Sena se thusa ditshebedisong tse kang tlhoekiso ya affinity, tlhahlobo ya dithethefatsi, kapa ntshetsopele ya biosensor.

Phetoho ea bokaholimo: NSP-SA-NHS e ka sebelisoa ho fetola bokaholimo, joalo ka li-slide tsa khalase kapa li-nanoparticles, ka liprotheine kapa li-peptide, ho theha libaka tse sebetsang ka biomolecule bakeng sa lits'ebetso tse fapaneng joalo ka tlhahlobo, litsamaiso tsa ho fana ka meriana, kapa li-platform tsa bio-sensing.