Ho na le bahale ba bangata ba sa bitsoeng ho re potoloha, ba bonahalang eka ke batho ba tloaelehileng, empa ha e le hantle ba kenya letsoho haholo ho rona ka khutso.Proteinase K ke "mohale ea sa tsejoeng" indastering ea tlhahlobo ea limolek'hule, leha e bapisoa le "e kholo le e matla" indastering, proteinase K e tlase haholo hoo esale re iphapanyetsa bohlokoa ba eona.Ka ho qhoma ha seoa se secha sa moqhaka, tlhokahalo ea proteinase K e eketsehile, mme phepelo ea lapeng le kantle ho naha e salletse morao haholo ts'ebeliso, mme ka tšohanyetso bohle ba hlokomela hore proteinase K e bohlokoa haholo.
Tšebeliso ea protheine K ke efe?
Proteinase K ke serine protease e nang le ts'ebetso ea enzyme ea proteolytic mme e ka boloka ts'ebetso libakeng tse ngata tse fapaneng (pH (4-12.5), buffer ea letsoai le phahameng, mocheso o phahameng oa 70 ° C, joalo-joalo).Ho phaella moo, mosebetsi oa proteinase K ha o thibeloe ke SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, joalo-joalo, 'me tekanyo e itseng ea sesepa e ka boela ea ntlafatsa mosebetsi oa proteinase K. Ka phekolo ea meriana (kokoana-hloko le likokoana-hloko tse sa tšoaneng ), lijo (ho nolofatsa nama), letlalo (ho nolofatsa moriri), ho etsa veine (ho hlakisa joala), ho lokisoa ha amino acid (masiba a senyehileng), ho ntšoa ha nucleic acid, in situ hybridization, joalo-joalo, proteinase K Ho na le lits'ebetso.Kopo e sebelisoang haholo ke nucleic acid extract.
Proteinase K e ka etsa enzymolyze mefuta eohle ea liprotheine tsa sampole, ho kenyeletsoa le li-histones tse tlamelletsoeng ka thata ho nucleic acid, e le hore li-nucleic acid li ka ntšoa sampoleng 'me li lokolloe ho qotsulo, ho thusa mohato o latelang oa ho ntšoa le tlhoekiso.Ha ho fumanoa varal nucleic acid, proteinase K ke e 'ngoe ea likarolo tsa bohlokoa tsa tharollo ea sampole ea vaerase.Proteinase K e ka senya le ho tima protheine ea seaparo sa kokoana-hloko, e sireletsehileng nakong ea lipalangoang le sethaleng sa ho lemoha;Ho feta moo, proteinase K e ka boela ea theola RNase e thibela ho senyeha ha kokoana-hloko ea RNA le ho thusa ho lemoha ha nucleic acid.
Botumo ba bosiu bo bong ba proteinase K
Ebang ke lefapheng la lipatlisiso tsa saense kapa lefapheng la IVD, ho ntšoa ha nucleic acid ke teko ea motheo ka ho fetisisa, kahoo proteinase K esale e le ntho ea bohlokoa haholo.Leha ho le joalo, nakong e fetileng, proteinase K e ne e sa tsejoe hakaalo ho feta karolo ea eona.Karolo e kholo ea sena e ne e le hobane kamano ea phepelo le tlhoko ea proteinase K e ne e tsitsitse haholo.Ke batho ba fokolang ba ka nahanang hore phepelo ea proteinase K e ka ba bothata.
Ka ho qhoma ha seoa se secha sa moqhaka, tlhokahalo ea tlhahlobo ea nucleic acid e eketsehile.Ho tloha bofelong ba Phuptjane 2020, China e phethile liteko tse ncha tsa moqhaka tse ka bang limilione tse 90, 'me palo ena e tšosa le ho feta lefatšeng ka bophara.Litekong tsa ho ntšoa ha nucleic acid, khatello e sebetsang ea proteinase K e ka ba 50-200 μg/mL.Ka kakaretso, ho nka 100 μg ea proteinase K ho ntša sampole ea nucleic acid.Ha e le hantle, e le ho eketsa katleho ea nucleic acid extract, hangata Proteinase K e tla sebelisoa ka tekanyo e eketsehileng.Ho sibolloa ha Nucleic acid ea coronavirus e ncha ho tlisitse palo e kholo ea tlhoko ea proteinase K.Phepelo ea mantlha le tlhokahalo e leka-lekaneng ea proteinase K e ile ea robeha kapele, 'me proteinase K ea fetoha sesebelisoa sa bohlokoa sa thibelo ea seoa ka bosiu bo le bong.
Mathata a ho hlahisa proteinase K
Le hoja ka tsoelo-pele ea seoa sena, boleng ba bohlokoa ba proteinase K bo 'nile ba nkoa e le ba bohlokoa ke batho, hoa hlajoa ke lihlong hore ka lebaka la linotlolo tse ngata tse tlaase tsa proteinase K, lik'hamphani tse seng kae tsa malapeng li kentse letsoho tlhahisong ea proteinase K. batla ho theha tlhahiso ea proteinase K Nakong ea ts'ebetso ea tlhahiso, ho ile ha sibolloa hore proteinase K ke protheine e ikhethang haholo.Ho thata haholo ho holisa bokhoni ba tlhahiso ea proteinase K ka nako e khuts'oane.
Tlhahiso e kholo ea proteinase K e tobane le mathata a latelang
1. Polelo e tlaase
Proteinase K e ka senya liprotheine tse ngata ka mokhoa o sa tobang, 'me ea baka chefo e tebileng ho sele ea moamoheli.Ka hona, boemo ba polelo ea proteinase K hangata bo tlase haholo.Ho hlahlojoa ha litsamaiso tsa polelo le mefuta e hlalosang haholo proteinase K hangata ho hloka nako e telele.
2. Mesaletsa ea li-pigment le li-nucleic acid
Ho belisoa ho hoholo ho hlahisa palo e kholo ea 'mala le masalla a "nucleic acid".Ho thata ho tlosa litšila tsena ka mokhoa o bonolo oa ho hloekisa, 'me ho hloekisa ho rarahaneng ho eketsa litšenyehelo le ho fokotsa sekhahla sa ho hlaphoheloa.
3. Ho hloka botsitso
Proteinase K ha e tsitsitse ka ho lekaneng, e ka iketsetsa enzymolyze, 'me ho thata ho e boloka ka mokhoa o tsitsitseng ho 37 ° C ka nako e telele ntle le moemeli ea sireletsang.
4. Ho bonolo ho na pula
Ha ho lokisoa phofo e omisitsoeng ea protheine K, e le ho etsa bonnete ba hore litaba tse tiileng tsa proteinase K ka har'a phofo e omisitsoeng ke e kholo, hoa hlokahala ho eketsa sesebelisoa se sireletsang se omisitsoeng ka bongata bo boholo, empa concentration ea proteinase K e fihla ho 20mg/mL le ho feta, ho bonolo Ho bokellana ho etsa pula, e tlisang mathata a maholo ho omisoa hoa protheine K ka lintho tse tiileng tse phahameng.
5. Matsete a maholo
Proteinase K e na le ts'ebetso e matla ea protease 'me e ka hlahisa liprotheine tse ling ka laboratoring ka hydrolyze.Ka hona, proteinase K e hloka libaka tse khethehileng tsa tlhahiso, lisebelisoa le basebetsi bakeng sa lipatlisiso le nts'etsopele le tlhahiso.
Tharollo ea XD BIOCHEM ea proteinase K
XD BIOCHEM e na le polelo ea protheine e butsoitseng le sethala sa tlhoekiso, 'me e na le boiphihlelo bo bongata polelong le tlhoekisong ea liprotheine tse kopanyang le ho ntlafatsa ts'ebetso ea tlhahiso.Ka ho theha ka potlako sehlopha sa lipatlisiso le nts'etsopele, ts'ebetso e kholo ea tlhahiso ea proteinase K e hlotsoe.Khoeli ea khoeli le khoeli ea phofo e omisitsoeng ka leqhoa e feta 30 KG.Sehlahisoa se na le ts'ebetso e tsitsitseng, ts'ebetso e phahameng ea enzyme, 'me ha ho na masala a cytochrome le nucleic acid.Rea u amohela ho ikopanya le XD BIOCHEM Fumana sephutheloana sa liteko (E-mail:sales@xdbiochem.comMohala: +86 513 81163739).
Litharollo tsa tekheniki tsa XD BIOCHEM li kenyelletsa
Ho sebelisoa likopi tse ngata tsa kopanyo ea plasmid, ho khethoa mefuta e phahameng ea polelo e nang le boemo ba 8g/L, e hlolang bothata ba boemo bo tlase ba polelo ea proteinase K.
Ka ho theha mokhoa oa ho hloekisa mehato e mengata, masala a cytochrome le nucleic acid ea proteinase K a ile a tlosoa ka katleho ka tlase ho boleng bo tloaelehileng.
Ka tlhahlobo e phahameng ea lisebelisoa tsa li-buffer formulations, ho ile ha khethoa sekheo se ka bolokang proteinase K ka mokhoa o tsitsitseng ho 37°C.
Li-buffers tsa ho hlahloba li hlola bothata ba hore proteinase K e bonolo ho e bokella le ho na pula e ngata haholo, 'me e rala motheo oa protheine K's high solid content e omisang-ho hoama.
XD BIOCHEM proteinase K sampole
Teko ea botsitso ea XD BIOCHEM proteinase K: ho ke ke ha e-ba le phetoho e kholo mosebetsing ka mor'a 80 d mocheso oa kamore.
Teko ea botsitso ea XD BIOCHEM proteinase K: ho ke ke ha e-ba le phetoho e kholo mosebetsing ka mor'a 80 d mocheso oa kamore.
Papiso ea phello ea tlhahiso ea nucleic acid ea XD BIOCHEM proteinase K le lihlahisoa tse hlolisanang.Ts'ebetsong ea ho ntšoa ha nucleic acid, XD BIOCHEM le competitive proteinase K li sebelisoa ka ho latellana.Bokhoni ba ho ntša XD BIOCHEM proteinase K bo holimo 'me boleng ba Ct ba mofuta o lebisitsoeng bo tlase.
Nako ea poso: Dec-31-2021