Leupeptin hemisulfate CAS:103476-89-7

Leupeptin hemisulfate e sebelisoa haholo biochemistry ea protheine bakeng sa ho boloka liprotheine tse reretsoeng nakong ea lits'ebetso tsa tlhoekiso. Ka ho eketsa leupeptin hemisulfate lisampoleng kapa li-buffer tsa protheine, bafuputsi ba ka thibela ts'ebetso ea protease le ho boloka botšepehi ba liprotheine, ho netefatsa tlhahlobo e nepahetseng le ts'ebeliso e tlase. Baelojing ea sele, leupeptin hemisulfate e bapala karolo ea bohlokoa ho thibeleng ho senyeha ha protheine nakong ea lits'ebetso tsa lysis ea sele. Ho kenyelletsoa ha protease inhibitor ena ho li-buffer tsa lysis ea sele ho thusa ho boloka liprotheine tsa sele, ho lumella bafuputsi ho ithuta maemo a tlhahiso ea protheine, liphetoho tsa kamora phetolelo, le likamano tsa protheine le protheine ntle le tšitiso ho tsoa ho li-protease tsa tlhaho. Ho feta moo, leupeptin hemisulfate e bohlokoa ho ithuteng litsela le lits'ebetso tse tsamaisoang ke protease ka har'a lisele. Bafuputsi ba ka sebelisa leupeptin hemisulfate ho thibela li-protease tse itseng ka khetho, ho hlakisa mesebetsi ea tsona mesebetsing e fapaneng ea sele joalo ka apoptosis, phetisetso ea lets'oao, le phetoho ea protheine. Ka kakaretso, leupeptin hemisulfate e sebetsa e le reagent ea bohlokoa lipatlisisong tsa baeloji, e fa bafuputsi sesebelisoa se tšepahalang sa ho laola ts'ebetso ea protease le ho boloka botsitso ba protheine. Ho qhibiliha ha eona ho matlafetseng le katleho ea thibelo ho e etsa karolo e feto-fetohang le ea bohlokoa haholo liprothokholong tse ngata tsa liteko tse reretsoeng ho senola mekhoa e rarahaneng ea biochemistry le baeloji ea sele.