Leupeptin CAS:24125-16-4

Leupeptin e sebelisoa haholo lilaboratoring tsa lipatlisiso bakeng sa tšireletso ea liprotheine khahlanong le ho senyeha ha liprotheine tse phelang nakong ea tlhoekiso le tlhahlobo ea protheine. Ka ho thibela ts'ebetso ea protease, leupeptin e thusa ho boloka botšepehi le botsitso ba liprotheine tse reretsoeng, e leng se lumellang bafuputsi ho fumana liphetho tse nepahetseng haholoanyane litekong tsa bona. Baelojing ea sele, leupeptin hangata e kenyelletsoa li-buffer tsa lysis ea sele ho thibela ho senyeha ha protheine nakong ea ho ntšoa ha liprotheine tsa sele. Sena se tiisa hore liprotheine tse ntšitsoeng liseleng li lula li sa senyeha ebile li sebetsa, e leng se nolofalletsang lits'ebetso tse latelang joalo ka Western blotting, liteko tsa enzyme, le lithuto tsa tšebelisano ea protheine le protheine. Ho feta moo, leupeptin e sebelisoa hangata lithutong tse batlisisang karolo ea li-protease tse itseng lits'ebetsong tsa sele. Ka ho thibela ts'ebetso ea protease ka leupeptin ka mokhoa o ikhethileng, bafuputsi ba ka hlakisa mesebetsi ea li-protease litseleng tsa lipontšo, phetoho ea protheine le lits'ebetso tse ling tsa 'mele. Ka kakaretso, leupeptin ke sesebelisoa sa bohlokoa lipatlisisong tsa biochemical le baeloji ea sele, e fa bafuputsi mokhoa o tšepahalang oa ho laola ts'ebetso ea protease le ho sireletsa liprotheine ho senyeha. Ho tenyetseha ha eona, botsitso le sekgahla se seholo sa thibelo ho e etsa hore e be sesebelisoa sa bohlokoa bakeng sa mefuta e fapaneng ea lits'ebetso tse reretsoeng ho utloisisa mekhoa e rarahaneng e thehiloeng lits'ebetsong tsa baeloji.