Theko ea Moetsi oa Iodoacetamide CAS:144-48-9

Phetoho ea Cysteine: Iodoacetamide e arabela le lihlopha tsa free thiol ka har'a masala a cysteine, e leng se etsang hore ho be le kamano e kopanetsoeng le sebabole sa cysteine. Phetoho ena e thusa tlhahlobong ea protheine ka ho thibela masala a cysteine ​​le ho thibela liketso tse sa lakatseheng kapa oxidation.

Ho Sila Protheine: Iodoacetamide hangata e sebediswa mesebetsing ya tshilo ya protheine bakeng sa tlhahlobo ya bongata ba spectrometry. E arabela le masalla a cysteine ​​pele ho tshilo ya enzyme, e thibela ho thehwa ha disulfide bond e sa batleheng le ho boloka protheine e le boemong ba yona bo fokotsehileng.

Lithuto tsa Proteomics: Iodoacetamide e sebelisoa maanong a ho ngola mabitso a kenyeletsang proteomics e thehiloeng ho mass spectrometry. Ka karabelo ea eona le masala a cysteine, e nolofalletsa ho ngola mabitso le ho khetholla li-peptide tse nang le cysteine.

Lithuto tsa Sebopeho sa Protheine: Iodoacetamide e sebelisoa lithutong tsa sebopeho ho fetola masala a cysteine ​​ka lik'hemik'hale le ho ithuta mosebetsi oa 'ona le ho kenella ha 'ona liphetohong tsa protheine tse menang, tse tlamang le tse fetohang sebopeho. E ka boela ea e-ba molemo litekong tsa ho hokahanya ho etsa 'mapa oa likamano tsa protheine le protheine.

Ho Kopanya Li-peptide tse Ngotsoeng ka Leibole: Iodoacetamide e ka sebelisoa ho kopanya li-peptide tse ngoliloeng ka ho fetola masala a cysteine ​​ka metsoako e ngoliloeng ka leibole ka isotopic, joalo ka deuterium kapa carbon-13. Li-peptide tsena tse ngoliloeng ka leibole li sebelisoa lits'ebetsong tse fapaneng tsa lipatlisiso, ho kenyeletsoa le ho lekanya protheine le lithuto tsa matla.