Genetic disulfate (G418 sulfate) CAS:108321-42-2

Libakeng tsa laboratori, geneticin disulfate (G418 sulfate) e sebelisoa hangata litekong tsa setso sa sele ho khetha le ho boloka mela ea sele e nang le li-vector tsa tlhahiso tse nang le liphatsa tsa lefutso tsa ho hanyetsa, joalo ka neomycin phosphotransferase. Lisele tsena li ka phela boteng ba geneticin disulfate ka lebaka la polelo ea gene ea ho hanyetsa, e leng se lumellang bafuputsi ho arola le ho ithuta baahi ba lisele tse fetotsoeng liphatsa tsa lefutso. Bafuputsi ba sebelisa geneticin disulfate lits'ebetsong tse fapaneng, ho kenyeletsoa tlhahiso e tsitsitseng ea lisele, mekhoa ea ho hlophisa liphatsa tsa lefutso joalo ka CRISPR-Cas9, le tlhahiso ea protheine e kopantsoeng. Ka ho kenyelletsa geneticin disulfate setsing sa setso, baahi ba itseng ba lisele ba ka ruisoa kapa ba aroloa ho latela bokhoni ba bona ba ho pholoha khetho ea lithibela-mafu. Ho ntlafatsa mahloriso le nako ea kalafo ea geneticin disulfate ho bohlokoa ho fihlela khatello e khethiloeng ha ho ntse ho fokotsoa chefo ea cytotoxicity le litlamorao tse sa lebelloang liseleng tse sa fetisetsoang. Ho beha leihlo ka hloko bophelo ba sele, litšobotsi tsa kholo, le mekhoa ea tlhahiso ea liphatsa tsa lefutso ho thusa ho netefatsa liphetho tse atlehileng tsa liteko ha ho sebelisoa geneticin disulfate lithutong tsa setso sa sele. Bafuputsi ba tlameha ho latela melaoana e thehiloeng bakeng sa tšebeliso ea geneticin disulfate, ho kenyeletsoa le mekhoa e loketseng ea ho lekanya, ho sebetsana le eona le ho e lahla, ho boloka maemo a polokeho a laboratori le ho khothaletsa liphetho tse nepahetseng tsa saense. Tšebelisano-'moho har'a bo-rasaense, basebetsi ba laboratori le balaoli ba taolo e tšehetsa tšebeliso e nang le boikarabelo le e sebetsang ea geneticin disulfate lipatlisisong tsa baeloji ea limolek'hule, e ntlafatsang kutloisiso ea rona ea lits'ebetso tsa sele le mekhoa ea ho laola liphatsa tsa lefutso.