Fluorescein Isothiocyante CAS:3326-32-7

Litšobotsi tsa khanya: FITC e na le chai e phahameng ea quantum 'me e ntša khanya e tala e khanyang ha e tsosoa hoo e ka bang 488 nm. Litšobotsi tsa eona tsa khanya li etsa hore e lokele ho fumanoa ho sebelisoa mekhoa e fapaneng ea ho nka litšoantšo, joalo ka microscopy ea khanya, cytometry ea phallo, le spectroscopy ea khanya.

Ho tlama ka covalent: FITC e na le sehlopha sa isothiocyanate se e lumellang ho tlama ka covalent ho lihlopha tsa amino tse teng liprotheineng, li-antibodies le limolek'hule tse ling. Ho tlama ka covalent ena ho netefatsa ho ngoloa ha limolek'hule tse reretsoeng ho tsitsisa le ho li bona ka mahlo.

Immunofluorescence: Li-antibodies tse nang le lebitso la FITC li sebelisoa hangata litekong tsa immunofluorescence ho lemoha li-antigen kapa liprotheine tse itseng liseleng le liseleng. Phatsimo e tala e khanyang e hlahisoang ke FITC e nolofalletsa bafuputsi ho fumana le ho bona ka mahlo a kelello kabo ea limolek'hule tse reriloeng ka har'a lisampole tsa baeloji.

Cytometry ea phallo: Li-antibodies tse nang le lebitso la FITC kapa li-biomolecule tse ling tse kopantsoeng ke fluorochrome li sebelisoa haholo cytometry ea phallo ho sekaseka le ho hlophisa lisele ho latela matšoao a tsona a bokaholimo kapa likarolo tsa ka hare ho sele. FITC e lumellana le laser ea 488 nm e sebelisoang hangata e sebelisoang lisebelisoeng tsa cytometry ea phallo.

Lithuto tsa tšebelisano-'moho ea protheine: FITC e ka sebelisoa lithutong tsa tšebelisano-'moho ea protheine ho lekola ho tlamahana le tšebelisano-'moho ea liprotheine. Ka ho ngola liprotheine tse itseng ka FITC, bafuputsi ba ka sekaseka likamano tsa bona ba sebelisa mekhoa e kang phetiso ea matla a fluorescence resonance (FRET) kapa polarization ea fluorescence.

Ho latela limolek'hule: FITC e ka sebelisoa ho latela le ho latela motsamao oa limolek'hule ka har'a lisele tse phelang kapa lisele tse phelang. Ka ho ngola limolek'hule tse khahlisang ka FITC, bafuputsi ba ka ithuta sebaka sa bona, matla a bona le mekhoa ea khoebo ka nako ea sebele ba sebelisa microscopy ea fluorescence.

Ditshebediso tsa tlhahlobo: Diprobe kapa di-antibodies tse ngotsweng ka leibole ya FITC di sebediswa ho hlahlobisiseng bakeng sa ditshebediso tse fapaneng, ho kenyeletswa le ho lemoha di-antigen tse itseng, dintho tse tshwaetsanang, kapa di-biomarker disampoleng tsa bakudi. Diprobe tsena tse tjhajang di fana ka tlhahlobo e bonolo le e ikgethang, e dumellang tlhahlobo e potlakileng le e nepahetseng.