DNase I, ho tsoa ho Bovine Pancreas CAS:9003-98-9

DNase I, e nkiloeng ho pancreas ea likhomo, ke enzyme e arolang limolek'hule tsa DNA ka ho li hydrolyze ka ho li kopanya le phosphodiester lesapong la mokokotlo la DNA. Tšusumetso e kholo ea DNase I ke ho senyeha le ho arohana ha DNA.

Tšebeliso e ka sehloohong ea DNase I ke lipatlisisong tsa baeloji ea limolek'hule. E sebelisoa hangata litekong tse fapaneng ho arola le ho hloekisa DNA, ho tlosa tšilafalo ea DNA ea genomic ho tsoa lisampoleng tsa RNA, le ho laola DNA. DNase I e sebetsa hantle haholo ho senyeng DNA metsoakong ea protheine-DNA, e lumellang bafuputsi ho ithuta likamano tsa protheine-DNA kapa ho tlosa liprotheine tse tlamiloeng ke DNA.

Ho phaella moo, DNase I e sebediswa kgafetsa ditekong tsa DNA, tse thusang ho hlwaya dibaka tsa DNA tse tlamilweng ke diprotheine tse itseng. Ka ho phekola sampole ya DNA ka DNase I le ho sekaseka dikotwana tsa DNA tse hlahang, bafuputsi ba ka fumana dibaka tsa ho tlama diprotheine tse tlamang DNA.

DNase I e boetse e sebelisoa tlhahlobong ea chromatin, moo e thusang ho sila chromatin (DNA le liprotheine tse amanang le eona) ho ithuta sebopeho le tlhophiso ea eona. Ka ho sila chromatin ka DNase I, bafuputsi ba ka khetholla libaka tse fumanehang habonolo le tse bonolo ho arohana, ba fana ka temohisiso mabapi le taolo ea liphatsa tsa lefutso le sebopeho sa chromatin.

Ho feta moo, DNase I e na le ditshebediso tsa tlhahlobo ya bongaka, haholoholo ho fumaneng le ho lekanya DNA e se nang disele mading a potolohang. Ka ho sebedisa DNase I ho sila DNA e sa potolohang, ho a kgoneha ho sekaseka le ho lemoha tatellano e itseng ya DNA e amanang le mafu kapa diphatsa tsa lefutso.