Disodium 4-nitrophenylphosphate CAS:4264-83-9

Tšusumetso e kholo le ts'ebeliso ea disodium 4-nitrophenylphosphate ke ts'ebeliso ea eona e le substrate ea chromogenic bakeng sa liteko tsa enzyme ea alkaline phosphatase. Ha motsoako ona o kopantsoe le alkaline phosphatase, enzyme e arola sehlopha sa phosphate ho disodium 4-nitrophenylphosphate, e leng se fellang ka ho thehoa ha 4-nitrophenol.

4-nitrophenol e hlahisitsoeng ke motsoako o mosehla o ka bonoang ka spectrophotometric ho 405 nm. Phetoho ena ea 'mala e sebelisetsoa ho lekanya ka bongata mosebetsi oa alkaline phosphatase lisampoleng, tse kang maro a baeloji kapa li-lysate tsa sele.

Tšebeliso ea disodium 4-nitrophenylphosphate litekong tsa enzyme e fana ka mokhoa o bonolo oa ho lekanya ts'ebetso ea alkaline phosphatase. Enzyme ena e ameha lits'ebetsong tse fapaneng tsa baeloji 'me hangata e sebelisoa e le letšoao la phapang ea lisele, ts'ebetso ea sebete le metabolism ea masapo.

Ho phaella moo, disodium 4-nitrophenylphosphate e ka sebediswa e le substrate bakeng sa di-phosphatase tse ding, tse kang acid phosphatase kapa di-protein phosphatase, ho itshetlehile ka setha sa teko le sepheo sa dipatlisiso.