Diethylene triamine pentaacetic acid CAS: 67-43-6

Diethylene triamine pentaacetic acid (DTPA) ke motsoako oa lik'hemik'hale o sebelisoang hangata e le moemeli oa chelating. E na le bokhoni ba ho kopana le li-ion tsa tšepe e boima, haholo-holo tse kang loto, mercury le cadmium, ho theha metsoako e tsitsitseng. DTPA e sebelisoa lits'ebetsong tse fapaneng, ho kenyeletsoa le tokiso ea tikoloho, kalafo ea bongaka, lits'ebetso tsa indasteri le meriana ea radiopharmaceuticals. Matla a eona a chelating ea tšepe a etsa hore e sebetse hantle ho tloseng litšepe tse boima metsing a litšila, ho phekola chefo ea tšepe e boima, ho thibela tšitiso liketsong tsa lik'hemik'hale, le ho nolofatsa ho fanoa ha meriana ea phekolo e reretsoeng. Ka kakaretso, DTPA ke motsoako o feto-fetohang o nang le lits'ebetso tsa bohlokoa liindastering tse fapaneng.

Odara kapa Kopa Khoteshene

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tšebeliso le Phello

Ho se sebetse ha Li-RNase: Morero o ka sehloohong oa DEPC ke ho se sebetse ha Li-RNase, e leng li-enzyme tse ka senyang RNA. DEPC e arabela le masala a histidine ho Li-RNase, e fetola libaka tsa tsona tse sebetsang le ho li etsa hore li se sebetse. Sena se netefatsa ho bolokoa ha botšepehi ba RNA nakong ea liteko tse kang ho itšehla thajana ha RNA, ho ngoloa ho reverse, le ho latellana ha RNA.

Ho boloka disampole tsa RNA: Kalafo ea DEPC hangata e sebelisoa lisebelisoa tsa laboratori, tse kang lipeipi tsa microcentrifuge, lintlheng tsa pipette, le metsi a sebelisoang litekong tsa RNA. Sena se thusa ho thibela li-RNase tse setseng tse ka bang teng le ho thibela ho senyeha ha RNA nakong ea ho sebetsana le ho boloka sampole.

Tokisetso ea reagent e se nang RNAse: DEPC e ka sebelisoa ho phekola litharollo kapa li-reagent tse hlokang ho ba le RNAse, joalo ka li-buffer, litharollo tsa Tris-EDTA (TE), kapa metsi a sebelisoang litekong tsa baeloji ea limolek'hule. Litharollo tsena tse phekotsoeng li ka sebelisoa ka mokhoa o sireletsehileng ho fokotsa ho senyeha ha RNA.

Phetoho ea DNA: DEPC e ka boela ea arabela le lihlopha tsa amino limolek'huleng tsa DNA, e leng se fellang ka phetoho ea carbethoxylated ea metheo ea DNA. Sena se ka sebelisoa lits'ebetsong tse itseng, joalo ka liketso tsa tatellano ea DNA kapa ho fetola DNA bakeng sa lithuto tsa ho hokahanya.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C6H10O5
Teko	99%
Ponahalo	Mokelikeli o se nang 'mala
Nomoro ea CAS	1609-47-8
Ho paka	E nyane le e ngata
Bophelo ba Shelofo	lilemo tse 2
Polokelo	Boloka sebakeng se pholileng le se omileng
Setifikeiti	ISO.