Concanavalin A CAS:11028-71-0 Theko ea moetsi

Concanavalin A (Con A) ke lectin, e leng mofuta oa protheine e ka kopanang ka ho khetheha le limolek'hule tse itseng tsa tsoekere. E nkiloe peong ea semela sa jackbean (Canavalia ensiformis).

Con A e na le kamano e phahameng bakeng sa masala a tsoekere a mannose le glucose mme e ka tlamahana le li-glycoprotein le li-glycolipid tse nang le tsoekere ena. E theha metsoako e ka fetoloang le limolek'hule tsena ka tšebelisano-'moho ea lik'habohaedreite le protheine.

Ka lebaka la bokhoni ba eona ba ho kopanya tsoekere, Con A e sebelisoa haholo lipatlisisong tsa baeloji le tlhahlobong. E 'nile ea sebelisoa haholo e le sesebelisoa sa ho ithuta li-glycoprotein, tsoekere ea bokaholimo ba sele, le likamano tse thehiloeng lik'habohaedreite. E ka sebelisoa ho arola le ho hloekisa li-glycoprotein ka ho sebelisa chromatography ea affinity, hammoho le ho lemoha le ho lekanya likarolo tse itseng tsa tsoekere holim'a bokaholimo ba sele kapa metsoakong e rarahaneng.

Con A e boetse e na le thepa ea ho laola sesole sa 'mele. E ka susumetsa ts'ebetso le ho ata ha lisele tse itseng tsa 'mele, tse kang li-lymphocyte tsa T, 'me e ithutiloe bakeng sa ts'ebeliso ea eona e ka bang teng ea phekolo kalafong ea 'mele le maano a ente.

Odara kapa Kopa Khoteshene

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tšebeliso le Phello

Ho Kopana ha Sele: E 'ngoe ea liphello tse hlahelletseng tsa Con A ke bokhoni ba eona ba ho baka ho kopana ha lisele. Thepa ena e bile molemo bakeng sa ho ithuta ho khomarela ha sele le likamano tsa bokaholimo ba sele.

Ho Ikarola ha Glycoprotein: Con A e tlama ka ho khetheha ho li-glycoprotein tse nang le mannose ea ho qetela le masala a glucose. Thepa ena e sebelisitsoe ho arola le ho hloekisa li-glycoprotein ho tsoa metswakong e rarahaneng, joalo ka maro a baeloji kapa li-lysate tsa sele, ka ho sebelisa chromatography ea Con A affinity.

Ho lemoha Liprotheine tsa Glyco: Con A e ka sebelisoa ho lemoha le ho lekanya liprotheine tsa glyco ka har'a lisampole. Ka ho sebelisa Con A e kopantsoeng le li-tag tsa fluorescent kapa enzyme, hoa khoneha ho bona kapa ho lekanya bongata ba liprotheine tsa glyco tse teng sampole ka microscopy ea fluorescence kapa mekhoa ea enzyme-linked immunosorbent assay (ELISA), ka ho latellana.

Ho Ngola Li-Labelle tsa Bokaholimo ba Sele: Ka lebaka la ho ratana ha eona le tsoekere ea bokaholimo ba sele, Con A e ka sebelisoa ho ngola mabitso a mefuta e itseng ea lisele kapa ho ithuta mekhoa ea glycosylation holim'a libaka tsa sele. Con A e kopantsoeng le li-fluorophore kapa li-tag tse ling e lumella pono le tlhahlobo ea lisele ka mekhoa e kang flow cytometry kapa fluorescence microscopy.

Ho fetola 'mele oa motho hore o se ke oa itšireletsa mafung: Con A e na le litšobotsi tsa ho fetola 'mele oa motho hore o se ke oa itšireletsa mafung 'me e ka ama ts'ebetso ea lisele tsa 'mele tsa ho itšireletsa mafung. E ka susumetsa ts'ebetso le ho ata ha li-lymphocyte tsa T, e leng se lebisang tlhahisong ea li-cytokine le likarabelo tse ling tsa 'mele oa ho itšireletsa mafung. Thepa ena e 'nile ea hlahlojoa kalafong ea 'mele ea ho itšireletsa mafung le nts'etsopele ea ente, moo Con A e 'nileng ea sebelisoa ho ntlafatsa likarabelo tsa 'mele oa ho itšireletsa mafung kapa ho fetola mamello ea 'mele ea ho itšireletsa mafung.

Sesebelisoa sa Patlisiso: Con A ke sesebelisoa se sebelisoang haholo mafapheng a fapaneng a lipatlisiso tsa baeloji. Se sebelisitsoe ho ithuta likamano tsa lik'habohaedreite le protheine, ho khomarela ha lisele, mekhoa ea glycosylation, le likarabelo tsa 'mele oa ho itšireletsa mafung. Bokhoni ba eona ba ho khomarela tsoekere e itseng bo e etsa hore e be ea bohlokoa bakeng sa ho ithuta le ho laola li-glycoprotein le li-glycolipid.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C15H24
Teko	99%
Ponahalo	Phofo e tšoeu
Nomoro ea CAS	11028-71-0
Ho paka	E nyane le e ngata
Bophelo ba Shelofo	lilemo tse 2
Polokelo	Boloka sebakeng se pholileng le se omileng
Setifikeiti	ISO.