4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

Karolo e ka Hare ea Enzyme: pNAG e sebelisoa haholo e le karolo e itseng ea lienzyme tse fapaneng tse amehang ho hydrolysis ea litlamo tsa β-D-glucoside. Ha lienzyme tsena li arola molek'hule ea pNAG, e ntša p-nitrophenol. Sena se lumella bafuputsi ho lekanya le ho lekanya ts'ebetso ea enzymatic.

Liteko tsa Mesebetsi ea Enzyme: Hydrolysis ea pNAG ke lienzyme tse itseng e ka bonoa le ho lekanyetsoa ka spectrophotometric. Sena se etsa hore pNAG e lokele liteko tsa mesebetsi ea enzyme, moo bongata ba p-nitrophenol e hlahisoang bo lekanang ka ho toba le ts'ebetso ea enzymatic.

Tlhahlobo ea Phepelo e Phahameng: pNAG e sebelisoa hangata litekong tsa tlhahlobo ea phepelo e phahameng ho khetholla le ho khetholla li-inhibitor tsa enzyme kapa li-activator. Ka ho hlahloba phello ea metsoako e fapaneng mosebetsing oa enzyme, bafuputsi ba ka khetholla bakhethoa ba meriana kapa li-modulator tsa ts'ebetso ea enzyme.

Lithuto tsa Pontšo ea Liphatsa tsa Lefutso: pNAG e boetse e sebelisoa lipatlisisong tsa baeloji ea limolek'hule ho ithuta polelo le taolo ea liphatsa tsa lefutso. Ka ho lekanya ts'ebetso ea enzymatic ea lienzyme tse itseng ho sebelisoa pNAG e le substrate, bafuputsi ba ka batlisisa phello ea polelo ea liphatsa tsa lefutso mosebetsing le ts'ebetsong ea lienzyme.