4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

Enzyme Substrate: pNAG e sebelisoa haholo joalo ka karoloana e ikhethileng bakeng sa li-enzyme tse fapaneng tse amehang ho hydrolysis ea li-bond tsa β-D-glucoside.Ha li-enzyme tsena li petsola molek'hule ea pNAG, e ntša p-nitrophenol.Sena se lumella bafuputsi ho lekanya le ho lekanya tšebetso ea enzymatic.

Litlhahlobo tsa Ts'ebetso ea Enzyme: Hydrolysis ea pNAG ka li-enzyme tse ikhethileng e ka bonoa 'me ea lekanyetsoa spectrophotometrically.Sena se etsa hore pNAG e tšoanelehe bakeng sa litlhahlobo tsa tšebetso ea enzyme, moo palo ea p-nitrophenol e hlahisoang e lekanang ka kotloloho le ts'ebetso ea enzymatic.

Tlhahlobo e Phahameng ka ho Fetisisa: pNAG e atisa ho sebelisoa litlhahlobong tsa tlhahlobo e phahameng ea katleho ho khetholla le ho khetholla li-enzyme inhibitors kapa activator.Ka ho lekola phello ea metsoako e fapaneng ts'ebetsong ea enzymatic, bafuputsi ba ka tseba batho ba ka bang teng ho sebelisa lithethefatsi kapa li-modulator tsa tšebetso ea enzyme.

Lithuto tsa Gene Expression: pNAG e boetse e sebelisoa lipatlisisong tsa baeloji ea limolek'hule ho ithuta polelo ea lefutso le taolo.Ka ho lekanya ts'ebetso ea enzymatic ea li-enzyme tse ikhethileng tse sebelisang pNAG joalo ka karoloana, bafuputsi ba ka batlisisa phello ea polelo ea liphatsa tsa lefutso tšebetsong ea enzyme le ts'ebetso.