3,3′,5,5′-Tetramethylbenzidine sulfate CAS:54827-18-8

ELISA: E sebelisoa haholo e le substrate bakeng sa horseradish peroxidase (HRP) litekong tsa ELISA. Ha ho na le molek'hule e reretsoeng, joalo ka antigen kapa antibody, enzyme HRP e susumetsa oxidation ea 3,3', 5,5'-Tetramethylbenzidine sulfate, e lebisang ho thehoeng ha sehlahisoa se seputsoa. ​​Matla a 'mala a lekana ka ho toba le mahloriso a molek'hule e reretsoeng, e leng se lumellang tlhahlobo ea bongata.

Liteko tsa ts'ebetso ea enzyme: 3,3', 5,5'-Tetramethylbenzidine sulfate e ka sebelisoa ho lekanya ts'ebetso ea enzyme. Ka ho kenya sampole ea enzyme ka substrate, karabelo ea enzyme e ka hlahlojoa ke phetoho ea 'mala. Ts'ebetso e phahameng ea enzyme e lebisa 'mala o moputsoa o matla haholoanyane, e leng se nolofalletsang ho fumana kinetics ea enzyme le lithuto tsa thibelo.

Immunohistochemistry: E ka sebelisoa bakeng sa mekhoa ea ho daea ho immunohistochemistry (IHC). E sebelisoa e le chromogen bakeng sa li-antibodies tsa mantlha kapa tsa bobeli tse nang le lebitso la horseradish peroxidase, e leng se fellang ka ho thehoa ha depositi e putsoa e bonahalang sebakeng sa tšebelisano ea antigen-antibody.

Patlisiso ea lik'hemik'hale: 3,3', 5,5'-Tetramethylbenzidine sulfate e fumana ts'ebeliso lithutong tse fapaneng tsa lipatlisiso tsa lik'hemik'hale, haholo-holo ho fumaneng le ho lekanya mesebetsi ea li-enzyme, likamano tsa protheine le protheine, le likamano tsa protheine le DNA.