3,3′,5,5′-Tetramethylbenzidine CAS:54827-17-7

Ho biochemistry le baeloji ea limolek'hule, 3,3', 5,5'-Tetramethylbenzidine e sebetsa e le sesebelisoa sa bohlokoa bakeng sa ho lemoha le ho lekanya ts'ebetso ea enzymatic, haholo-holo ea peroxidases. Ha TMB e oxidized ke peroxidase moo ho nang le hydrogen peroxide, sehlahisoa sa chromogenic se 'mala o moputsoa sea hlahisoa, se lumellang pono le ho lekanya karabelo ea enzymatic. Thepa ena e etsa hore TMB e be sesebelisoa sa bohlokoa litekong tse fapaneng tsa immunoassay, ho kenyeletsoa ELISA, moo e sebelisoang khafetsa ho lemoha boteng ba li-antigen kapa li-antibodies tse itseng lisampoleng tsa baeloji. Balateli ea colorimetric e fanoeng ke TMB e nolofalletsa bafuputsi le litsebi tsa tlhahlobo ho lekola mahloriso a limolek'hule tse reriloeng ka nepo, e leng se tlatsetsang kutloisisong ea lits'ebetso tsa lefu, ho sibolloa ha biomarker le liteko tsa tlhahlobo. Ho feta moo, TMB e fumana lits'ebetso ho tlhahlobo ea bongaka, moo karabelo ea eona ea colorimetric e nolofalletsang ho lemoha li-biomarker, li-agent tse tšoaetsanoang le lintlha tsa autoimmune. Ka ho sebelisa bokhoni ba TMB ba ho hlahisa phetoho e bonahalang ea 'mala boteng ba li-analyte tse itseng le ts'ebetso ea peroxidase, litsebi tsa tlhokomelo ea bophelo li ka etsa liteko tse potlakileng le tse bonolo bakeng sa maemo a fapaneng, ho tloha mafung a tšoaetsanoang ho ea ho matšoao a kankere. Ho feto-fetoha ha TMB litekong tsa bongaka ho tšehetsa nts'etsopele ea lisebelisoa tsa tlhahlobo le liforomo tse sebelisoang lilaboratoring tsa bongaka, libakeng tsa tlhokomelo ea bakuli le litsi tsa lipatlisiso. Ho phaella moo, TMB e sebelisoa lipatlisisong tsa biotechnology le meriana bakeng sa tlhahlobo ea bongata le boleng ba likamano tsa protheine le protheine, kinetics ea enzymatic, le ho sibolloa ha lithethefatsi. Ho lumellana ha eona le litsamaiso tse iketsang le li-microplate readers ho lumella tlhahlobo le tlhahlobo ea ho feta tekano, ho thusa ho ithuta litsela tsa biochemical, likamano tsa receptor-ligand, le tlhahlobo ea metsoako ea phekolo. Tšebeliso ea TMB e atoloha ho ea tlhahlobong ea thibelo ea enzymatic le tlhaloso ea liprotheine tse kopantsoeng, e tlatsetsang tsoelo-peleng ea lithethefatsi le kutloisiso ea mekhoa ea limolek'hule e bakang mafu. Qetellong, 3,3', 5,5'-Tetramethylbenzidine e bapala karolo ea bohlokoa lipatlisisong tsa biochemical le tsa bongaka, e sebetsa e le substrate e feto-fetohang le e bonolo bakeng sa ho lemoha mebala litekong tsa 'mele, ELISA, tlhahlobo ea bongaka le ho sibolloa ha meriana. Bokhoni ba eona ba ho hlahisa phetoho e tsitsitseng le e ka bonoang ea 'mala boteng ba ts'ebetso ea peroxidase bo nolofalletsa litekanyo tse nepahetseng le tse sebetsang tsa likamano tsa biomolecular, mesebetsi ea enzymatic, le matšoao a amanang le lefu. Tšebeliso e pharaletseng ea TMB e totobatsa bohlokoa ba eona ho ntšetseng pele lipatlisiso tsa saense le tsa bongaka, e kenya letsoho nts'etsopele ea mahlale a tlhahlobo, mekhoa ea phekolo, le kutloisiso ea rona ea lits'ebetso tse rarahaneng tsa baeloji. Phuputso e tsoelang pele le ntlafatso ea liteko tse thehiloeng ho TMB ho ka lebisa ho boqapi bo eketsehileng le ntlafatso lipatlisisong tsa bongaka le tlhahlobo ea bongaka.