3,3 ,5,5 -Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

3,3', 5,5'-Tetramethylbenzidine dihydrochloride (TMB) ke substrate e sebediswang haholo ditekong tsa immunosorbent tse amanang le enzyme (ELISAs) le ditekong tse ding tsa biochemical. Ha TMB e oxidiswa ke enzyme horseradish peroxidase (HRP) moo ho nang le hydrogen peroxide, e hlahisa mmala o moputsoa. ​​Phetoho ena ya mmala e sebedisetswa ho lemoha boteng ba di-antibodies tse ngotsweng ka HRP kapa diprotheine tse ding, hangata e bontsha boteng ba antigen e itseng sampole. Mmala o moputsoa o hlahiswang ke karabelo hangata o lekanngwa ho sebediswa spectrophotometer, e dumellang ho lekanya matla a phetoho ya mmala mme e fana ka mokgwa wa ho hokahanya matla a mmala le bongata ba molek'hule e rerilweng e teng sampoleng. TMB e na le ditshebediso tse ngata ditekong tsa tlhahlobo, dipatlisisong le ntshetsopeleng ya meriana, haholoholo ho fumaneng di-antibodies, dihormone, di-cytokine le di-biomolecule tse ding. Kutloisiso ya yona, botsitso, le bonolo ba tshebediso di e etsa sesebediswa sa bohlokwa dibakeng tse fapaneng tsa laboratori bakeng sa ho lemoha le ho lekanya dimolek'hule tse itseng.