3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

Ke probe ea fluorescent e nang le sehlopha sa nitrobenzoxadiazole (NBD), se nang le thepa e matla ea fluorescence. Sehlopha sa aldehyde se lumella ho ngoloa le ho fumanoa ha limolek'hule tse itseng tse kang liprotheine, li-nucleic acid le lipids.

NBD-aldehyde hangata e sebediswa e le dae e arabelang ditekong bakeng sa ho lemoha le ho lekanya dikarabelo tsa ho kopanya protheine kapa peptide, diphetoho tsa protheine, le dikamano tsa protheine le protheine. E ka ngola dihlopha tsa amino tse lokolohileng diprotheineng kapa dipeptide, ya etsa dibopeho tse tsitsitseng tsa covalent. Sena se etsa hore e be molemo bakeng sa ditshebediso tse kang immunocytochemistry, immunohistochemistry, le western blotting.

Phatsimo ea NBD-aldehyde e ka tsosoa ke khanya ea ultraviolet kapa e putsoa, ​​​​'me e ntša khanya e tala. Thepa ena e nolofalletsa ho bona le ho lekanya li-biomolecule tse ngotsoeng ka ho sebelisa mekhoa ea fluorescence microscopy kapa spectroscopy.