TRIS-Acetate CAS: 6850-28-8 Theko ea Moetsi

Tris-acetate (TRIS-Acetate) ke buffer e sebelisoang hangata litekong tsa baeloji le tsa biochemical.E na le motsoako oa tris(hydroxymethyl)aminomethane (Tris) le acetic acid, e sebetsang e le molaoli oa pH le stabilizer.PH ea TRIS-Acetate buffer hangata e tloha ho 7.4 ho isa ho 8.4.
Phello e kholo ea TRIS-Acetate ke ho boloka pH e tsitsitseng, e leng bohlokoa bakeng sa karabelo e mengata ea likokoana-hloko le lik'hemik'hale.E sebetsa e le tšireletso ka ho fokotsa liphetoho leha e le life tsa bohlokoa ho pH tse ka hlahang ka lebaka la li-acids tse ekelitsoeng kapa metheo nakong ea liteko.
TRIS-Acetate e fumana lits'ebetso tse fapaneng ho biology ea limolek'hule, biochemistry, le biotechnology:
DNA le RNA Electrophoresis: TRIS-Acetate e atisa ho sebelisoa e le buffer e sebetsang ho agarose le polyacrylamide gel electrophoresis.E fana ka tikoloho e tsitsitseng ea pH nakong ea karohano ea likaroloana tsa DNA le RNA ho latela boholo ba tsona.
Tlhahlobo ea Liprotheine: Li-buffers tsa TRIS-Acetate li sebelisoa bakeng sa protheine electrophoresis, tse kang SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).E netefatsa botsitso ba protheine le karohano nakong ea ts'ebetso.
Karabelo ea Enzyme: Li-buffer tsa TRIS-Acetate li sebelisoa khafetsa litekong le lithutong tsa enzyme.E fana ka mofuta o nepahetseng oa pH bakeng sa karabelo e fapaneng ea enzymatic mme e thusa ho boloka ts'ebetso ea enzyme.
Cell and Tissue Culture: TRIS-Acetate buffers e sebelisoa mecheng ea litaba tsa cell culture ho boloka pH e nepahetseng bakeng sa kholo ea lisele le ho ata.E thusa ho boloka maemo a fisioloji a hlokehang bakeng sa ho phela ha sele.