IPTG CAS: 367-93-1 Theko ea Moetsi
Isopropyl β-D-1-thiogalactopyranoside (IPTG) ke analoge ea maiketsetso ea lactose e sebelisoang hangata liphuputsong tsa baeloji ea limolek'hule le lits'ebetsong tsa biotechnology.IPTG e sebelisoa haholo-holo ho susumetsa tlhahiso ea liphatsa tsa lefutso tsamaisong ea baktheria, moo e sebetsang e le sesosa sa molek'hule ho qala ho ngoloa ha liphatsa tsa lefutso.
Ha e kenyelelitsoe mocheng oa ho hōla, IPTG e nkoa ke libaktheria 'me e ka tlama protheine ea lac repressor, e thibela ho thibela mosebetsi oa lac operon.Lac operon ke sehlopha sa liphatsa tsa lefutso tse amehang ho metabolism ea lactose, 'me ha protheine e hatellang e tlosoa, liphatsa tsa lefutso li hlahisoa.
IPTG hangata e sebelisoa mmoho le lacUV5 mutant promoter, e leng mofuta o sebetsang oa mokhatlalatsi oa lac.Ka ho kopanya tlhahiso ea IPTG le mothetsi enoa ea feto-fetohang, bafuputsi ba ka fihlela maemo a phahameng a polelo ea lefutso.Sena se lumella ho hlahisa liprotheine tse ngata bakeng sa tlhoekiso kapa lisebelisoa tse ling tse tlaase.
Ntle le polelo ea liphatsa tsa lefutso, IPTG e boetse e sebelisoa khafetsa litekong tsa tlhahlobo e putsoa / e tšoeu.Mohlaleng ona, mofuta oa lacZ hangata o kopantsoe le lefutso la thahasello, 'me libaktheria tse atlehang ho hlahisa mofuta ona oa motsoako li tla hlahisa enzyme e sebetsang ea β-galactosidase.Ha IPTG e eketsoa hammoho le substrate ea chromogenic joalo ka X-gal, libaktheria tse hlahisang mofuta oa fusion li fetoha boputsoa ka lebaka la tšebetso ea β-galactosidase.Sena se lumella ho khetholla le ho khethoa ha mefuta e kopanetsoeng e kopantsoeng ka katleho le lefutso la thahasello.
Induction of gene expression: IPTG e atisa ho sebelisoa ho susumetsa polelo ea liphatsa tsa lefutso tsa liphatsa tsa lefutso tsamaisong ea baktheria.E etsisa lactose ea tlhaho ea inducer mme e tlama ho protheine ea lac repressor, e e thibela ho thibela lac operon.Sena se lumella ho ngoloa le ho hlahisa liphatsa tsa lefutso tse lakatsehang.
Polelo ea protheine le tlhoekiso: Induction ea IPTG e atisa ho sebelisoa ho hlahisa liprotheine tse ngata tse kopantsoeng bakeng sa merero e fapaneng, e kang lithuto tsa lik'hemik'hale, tlhahiso ea phekolo, kapa tlhahlobo ea moralo.Ka ho sebelisa li-vector tse nepahetseng tsa polelo le induction ea IPTG, bafuputsi ba ka fihlela maemo a holimo a tlhahiso ea protheine ho lihlopha tsa baktheria.
Tlhahlobo e putsoa / e tšoeu: IPTG e sebelisoa khafetsa hammoho le mofuta oa lacZ le substrate ea chromogenic, joalo ka X-gal, bakeng sa liteko tsa tlhahlobo e putsoa / e tšoeu.Mofuta oa lacZ hangata o kopantsoe le lefutso la thahasello, 'me libaktheria tse atlehang ho hlahisa mofuta ona oa motsoako li tla hlahisa enzyme e sebetsang ea β-galactosidase.Ha IPTG le substrate ea chromogenic li eketsoa, li-recombinant strains tse hlalosang mofuta oa fusion li fetoha boputsoa, ho lumella ho tsebahatsa habonolo le khetho.
Boithuto ba taolo ea liphatsa tsa lefutso: Induction ea IPTG hangata e sebelisoa lipatlisisong tsa ho ithuta taolo ea liphatsa tsa lefutso le li-operens, haholo-holo lac operon.Ka ho laola maikutlo a IPTG le ho lekola ponahalo ea likarolo tsa lac operon, bafuputsi ba ka etsa lipatlisiso ka mekhoa ea taolo ea liphatsa tsa lefutso le karolo ea lintlha kapa liphetoho tse fapaneng.
Sistimi ea polelo ea liphatsa tsa lefutso: IPTG ke karolo ea bohlokoa lits'ebetsong tse 'maloa tsa polelo ea liphatsa tsa lefutso, joalo ka litsamaiso tse thehiloeng ho batšehetsi ba T7.Lits'ebetsong tsena, mokhatlalatsi oa lac o atisa ho sebelisoa ho khanna polelo ea T7 RNA polymerase, eo, le eona, e ngolang liphatsa tsa lefutso tse lebisitsoeng tlas'a taolo ea tatellano ea li-promota tsa T7.IPTG e sebelisoa ho susumetsa polelo ea T7 RNA polymerase, e lebisang ts'ebetsong ea polelo ea gene ea sepheo.
| Sebopeho | C9H18O5S |
| Tlhahlobo | 99% |
| Ponahalo | Phofo e tšoeu |
| Nomoro ea CAS. | 367-93-1 |
| Ho paka | Tse nyenyane le tse ngata |
| Shelf Life | lilemo tse 2 |
| Polokelo | Boloka sebakeng se phodileng le se omileng |
| Setifikeiti | ISO. |
