5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ke motsoako o sebelisoang lithutong tse fapaneng tsa biochemical, haholo-holo bakeng sa ho lemoha le ho bona tšebetso ea enzyme.Ke substrate e ka kenngoa ka hydrolyzed ka li-enzyme tse itseng, e leng se bakang ho lokolloa ha sehlahisoa se mebala kapa sa fluorescent.

Motsoako ona o sebelisoa hangata litekong tsa ho bona boteng le tšebetso ea li-enzyme tse kang beta-galactosidase le beta-glucuronidase.Li-enzyme tsena li qhekella lihlopha tsa acetyl le glucosaminide ho tloha substrate, e leng se lebisang ho thehoeng ha chromophore e putsoa kapa e tala.

Sebopeho se ikhethileng sa 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide se lumella ho lemoha habonolo le ho lekanya mosebetsi oa enzyme.Tšebeliso ea eona mekhoeng e fapaneng ea liteko, ho kenyelletsa histochemistry, immunohistochemistry, le litlhahlobo tse thehiloeng liseleng, e tlatselitse kutloisisong e ntle ea mesebetsi ea enzyme.

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5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) ke substrate ea chromogenic e atisang ho sebelisoa bakeng sa ho lemoha mosebetsi oa beta-glucuronidase (GUS).GUS ke enzyme e fumanehang linthong tse fapaneng, ho kenyelletsa libaktheria, limela le liphoofolo tse anyesang.X-Gluc e sebelisoa khafetsa litekong tsa moqolotsi oa litaba oa GUS le lipatlisisong tsa baeloji ea limolek'hule.

Tšebeliso e ka sehloohong ea X-Gluc ke mekhoa ea ho silafatsa histochemical, moo e ka bonang ka mahlo a kelello polelo le ts'ebetso ea enzyme ea GUS.Substrate ena e khona ho kenella ka liseleng 'me e fetoha hydrolyzed ke GUS, e leng se etsang hore ho thehoe sehlahisoa se putsoa kapa se ke keng sa qhibiliha.Lebala lena le leputsoa le lumella bafuputsi ho tseba le ho beha tšebetso ea GUS liseleng, liseleng le linthong tse phelang kaofela.

X-Gluc e ka boela ea sebelisoa litekong tsa bongata ho lekanya ts'ebetso ea enzyme ea GUS.Matla a 'mala o moputsoa kapa bongata ba sehlahisoa se entsoeng bo ka amahanngoa le boemo ba polelo ea GUS kapa mosebetsi oa eona oa enzymatic.

Ho feta moo, X-Gluc e hiriloe lipatlisisong tsa lefutso la limela ho ithuta polelo ea liphatsa tsa lefutso, ts'ebetso ea batšehetsi le phetoho ea limela.E boetse e sebelisitsoe lits'ebetsong tsa baktheria bakeng sa ho etsa cloning le ho lemoha liprotheine tsa GUS fusion.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C16H18BrClN2O6
Tlhahlobo	99%
Ponahalo	Phofo e tšoeu
Nomoro ea CAS.	4264-82-8
Ho paka	Tse nyenyane le tse ngata
Shelf Life	lilemo tse 2
Polokelo	Boloka sebakeng se phodileng le se omileng
Setifikeiti	ISO.